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宿主来源
Rat抗原名称
CD324分子别名
Cadherin-1; CAM 120/80; Epithelial cadherin (E-cadherin); Uvomorulin; CDHE; UVO; CDH1细胞定位
Cell membrane, Cytoplasm, EndosomeAccession
P12830克隆号
S-R650抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Ms阳性样本
HT-29纯化方式
Protein G浓度
0.05mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu, Ms
CD324, also known as E-cadherin or cadherin-1, is a calcium-dependent cell adhesion protein that plays a crucial role in maintaining epithelial tissue integrity and regulating cell proliferation, differentiation, and survival. It is a member of the cadherin superfamily and is widely expressed on the cell surface of most epithelial tissues. The extracellular domain of CD324 facilitates homophilic binding between adjacent cells, while its cytoplasmic domain interacts with the actin cytoskeleton through catenins. This protein is essential for epithelial cell cohesion and acts as an invasion suppressor, with its loss often indicating high tumor aggressiveness in cancer.
流式分析
Flow cytometric analysis of CD324 expression on HT-29 cells. Cells from the HT-29 (Human colorectal adenocarcinoma epithelial cell, Right) or MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) was stained with either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Rat Anti-Mouse/Human CD324 Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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