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宿主来源
Rat抗原名称
CD324分子别名
Cadherin-1; CAM 120/80; Epithelial cadherin (E-cadherinImported); Uvomorulin; CDHE; UVO; CDH1细胞定位
Endosome, Cell membrane, CytoplasmAccession
P12830, P09803克隆号
S-R650抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms阳性样本
HT29纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:2000 Hu, Ms
CD324, also known as E-cadherin or cadherin-1, is a calcium-dependent cell adhesion protein that plays a crucial role in maintaining epithelial tissue integrity and regulating cell proliferation, differentiation, and survival. It is a member of the cadherin superfamily and is widely expressed on the cell surface of most epithelial tissues. The extracellular domain of CD324 facilitates homophilic binding between adjacent cells, while its cytoplasmic domain interacts with the actin cytoskeleton through catenins. This protein is essential for epithelial cell cohesion and acts as an invasion suppressor, with its loss often indicating high tumor aggressiveness in cancer.
流式分析
Flow cytometric analysis of MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) / HT29 (Human colorectal adenocarcinoma epithelial cell, Right) labelling CD324 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rat IgG1, κ Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: MDA-MB-231
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