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Armenian hamster Anti-Mouse CD194 Antibody (S-R694)

C-C chemokine receptor type 4,C-C CKR-4,CC-CKR-4,CCR-4,CCR4,Cmkbr4,Ccr4

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B5169
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Armenian hamster

  • 抗原名称

    CD194

  • 分子别名

    C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4; CCR-4; CCR4; Cmkbr4; Ccr4

  • 细胞定位

    Cell membrane

  • Accession

    P51680

  • 克隆号

    S-R694

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein G

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS pH7.4

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

  • 应用

    FCM

  • 稀释度

    应用 稀释度 推荐种属
    FCM 1:2000 Ms
背景介绍

  • CD194, also known as CCR4 (C-C chemokine receptor type 4), is a G protein-coupled receptor with seven transmembrane domains. It is expressed on various immune cells, including CD4+ T cells (especially Th2 cells), regulatory T cells (Tregs), basophils, monocytes, and natural killer (NK) cells. CCR4 binds to chemokines such as CCL17 (TARC) and CCL22 (MDC), playing a crucial role in the recruitment of memory T cells and immune responses in conditions like atopic dermatitis and asthma. Additionally, CCR4 is involved in tumor biology, with high expression levels associated with tumor infiltration and prognosis in both hematological malignancies and solid tumors, making it a potential target for cancer immunotherapy.

  • 免疫印迹

    • WB result of ××× Rabbit mAb
      Primary antibody: ××× Rabbit mAb at 1/××× dilution
      Lane 1: ××× whole cell lysate 20 µg
      Lane 2: ××× whole cell lysate 20 µg
      Lane 3: ××× whole cell lysate 20 µg
      Negative control: ××× whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: ××× kDa
      Observed MW: ××× kDa

  • 流式分析

    • Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD194 antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with an Armenian hamster IgG Isotype Control / (Left panel). Goat Anti-Armenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Cells were stained with CD3 - Alexa Fluor® 647 Antibody separately.

  • 免疫沉淀

    • ××× Rabbit mAb at 1/×× dilution (1 µg) immunoprecipitating ××× in 0.4 mg ××× whole cell lysate.
      Western blot was performed on the immunoprecipitate using××× Rabbit mAb at 1/××× dilution.
      Secondary antibody (HRP) for IP was used at 1/××× dilution.
      Lane 1: ××× whole cell lysate 20 µg (Input)
      Lane 2: ××× Rabbit mAb IP in ××× whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in ××× whole cell lysate
      Predicted MW: ××× kDa
      Observed MW: ××× kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human ×××. Anti-××× antibody was used at 1/××× dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded ×××. Anti-××× antibody was used at 1/××× dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded ×××. Anti-××× antibody was used at 1/××× dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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