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Mouse抗原名称
CD133分子别名
Prominin-1; Antigen AC133; Prominin-like protein 1; PROML1; PROM1细胞定位
Cell membrane, Endoplasmic reticulumAccession
O43490克隆号
S-R586抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein G浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD133, also known as prominin-1, is a pentaspan transmembrane glycoprotein encoded by the PROM1 gene. It is widely used as a biomarker for the identification and isolation of stem and progenitor cells in various normal tissues and cancer stem cells (CSCs) in different types of tumors. CD133 has five transmembrane domains, with its N-terminus extending into the extracellular space and the C-terminus residing inside the cell. It is expressed in neural progenitor cells, hematopoietic cells, and various cancer cells, including those in brain, colon, and pancreatic tumors. The presence of CD133 is associated with self-renewal, tumorigenicity, and resistance to chemotherapy and radiotherapy, making it a significant marker for cancer prognosis and therapeutic targeting.
流式分析
Flow cytometric analysis of Human CD133 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with PE/Cy7 Mouse Anti-Human CD45 Antibody and either FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD133 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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