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Rat抗原名称
CD45R/B220分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc细胞定位
Cell membrane, SynapseAccession
P06800, P08575克隆号
RA3-6B2抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
CD45R/B220 is an isoform of the CD45 protein, specifically expressed on murine B-lineage cells, including pro-B, pre-B, and mature B cells, as well as a small subset of T cells and NK cells. It is a 220 kDa surface molecule and a member of the protein tyrosine phosphatase (PTP) family, which plays a crucial role in regulating lymphocyte development and antigen receptor signaling. B220 is commonly used as a pan B-cell marker in mice due to its restricted expression on B cells, making it a valuable tool in immunological research and flow cytometry.
流式分析
Flow cytometric analysis of CD45R/B220 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either Biotin Isotype Control (Left panel) or SDT Biotin Rat Anti-Mouse/Human CD45R/B220 Antibody (Right panel) at 5μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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