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宿主来源
Rat抗原名称
CD49f分子别名
Integrin alpha-6; CD49 antigen-like family member F; VLA-6; ITGA6细胞定位
Cell membraneAccession
P23229, Q61739克隆号
GoH3抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
RhMk, CyMk, Hu, Ms阳性样本
Human peripheral blood lymphocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu, Ms
CD49f, also known as integrin α6 subunit, is a type I transmembrane glycoprotein encoded by the ITGA6 gene. It belongs to the α-subfamily of integrins and can form heterodimers with β1 (CD29) or β4 (CD104) subunits to create functional receptors such as α6β1 or α6β4. These receptors play crucial roles in cell adhesion, migration, and signaling. CD49f is highly expressed in various cell types, including T cells, monocytes, platelets, epithelial cells, and certain stem cells. In cancer biology, CD49f has been identified as a marker for tumor-initiating cells (TICs) in hepatocellular carcinoma (HCC), where it is associated with cell stemness, immune evasion, and the formation of an immunosuppressive tumor microenvironment. Additionally, CD49f has been used as a marker for human astrocytes and has shown potential in studying neurodegenerative diseases.
流式分析
Flow cytometric analysis of CD49f expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse/Human CD49f Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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