分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
CD73分子别名
5'-nucleotidase; 5'-NT; 5'-deoxynucleotidase; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase; NT5E; NT5; NTE免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P21589克隆号
S-715-115抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
NT5E (CD73) is a surface enzyme which is expressed on multiple cells. This enzyme mediates the gradual hydrolysis of the autocrine and paracrine danger signals of ATP and ADP to anti-inflammatory adenosine. Immune suppression mediated by adenosinergic pathways is very important for maintaining immune system homeostasis. Immune suppressive functions of T regulatory cells are also dependent on CD73 expression. CD73 also occurs on anergic CD4 + T cells, thereby maintaining self-tolerance to healthy tissues as well as protecting the fetus from the mother's immune system during pregnancy.
流式分析
Flow cytometric analysis of Human CD73 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either FITC Mouse IgG2b, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD73 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)