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产品规格
宿主来源
Rat抗原名称
Mouse CD19分子别名
B-lymphocyte antigen CD19; Differentiation antigen CD19细胞定位
Cell membraneAccession
P25918克隆号
S-R532抗体类型
Rat mAb抗体同种型
IgG2a应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
APC-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
稀释度
应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 1.25μl per million cells in 100μl volume | Ms |
背景介绍
CD19 is a 95 kd transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is a type I transmembrane protein with a single transmembrane domain, a cytoplasmic C-terminus, and an extracellular N-terminus. CD19 is crucial for B cell development and signaling as it modulates both B cell receptor (BCR)-dependent and independent signaling pathways. It is involved in the humoral immune response and tolerance induction, functioning as a critical co-receptor that amplifies the activation of Src-family protein tyrosine kinases.
流式分析
Flow cytometric analysis of Mouse CD19 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with either APC-Cy7 Rat IgG2a, κ Isotype Control (Black line histogram) or SDT APC-Cy7 Rat Anti-Mouse CD19 Antibody (Red line histogram) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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