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Mouse抗原名称
Mouse H-2Kb分子别名
H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K1; H2-K细胞定位
MembraneAccession
P01901克隆号
S-R659抗体类型
Mouse mAb抗体同种型
IgG2a,k应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein A浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1.25μl per million cells in 100μl volume | Ms |
H-2Kb protein is a major histocompatibility class I molecule expressed in mice, playing a crucial role in antigen presentation and immune response. It is involved in the processing and presentation of endogenous and exogenous peptide antigens via the MHC class I pathway, facilitating T cell-mediated cytotoxicity. H-2Kb is also implicated in the regulation of local cortical circuits in the brain, with its absence leading to excess connectivity that can function as a substrate for cortical plasticity. Additionally, H-2Kb has been studied in the context of viral infections, such as vesicular stomatitis virus, where it forms complexes with viral peptides and beta-2 microglobulin, providing insights into the structural basis of antigen recognition.
流式分析
Flow cytometric analysis of Mouse H-2Kb expression on C57BL/6 mouse splenocytes. Cells from the C57BL/6 mouse splenocytes (Right) or BALB/c mouse splenocytes (Left) were stained with either FITC Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT FITC Mouse Anti-Mouse H-2Kb Antibody (Red line histogram) at 1.25μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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