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宿主来源
Rat抗原名称
Mouse CD226分子别名
CD226 antigen; Platelet and T-cell activation antigen 1; Pta1细胞定位
Cell membraneAccession
Q8K4F0克隆号
S-R625抗体类型
Rat mAb抗体同种型
IgG2b,k应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:200 | Ms |
CD226, also known as DNAM-1, is a type I transmembrane protein belonging to the immunoglobulin superfamily and is widely expressed on the surface of immune cells, including T cells, natural killer (NK) cells, and B cells. It plays a crucial role in immune regulation by interacting with its ligands, CD155 and CD112. CD226 enhances immune cell adhesion, cytotoxic activity, and cytokine secretion upon activation. It is particularly important in antitumor immunity, where it competes with TIGIT and CD96 for ligand binding, thereby promoting the antitumor activity of immune cells. However, its function can be inhibited by the high-affinity binding of TIGIT in the tumor microenvironment. Additionally, CD226 is involved in modulating the function of regulatory T cells (Tregs), influencing immune tolerance and autoimmune responses.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD226 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Rat IgG2b, κ Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8α - Alexa Fluor® 647 Antibody separately.
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