FITC Rat Anti-Mouse/Human CD45R/B220 Antibody (S-R519)
CD45,PTPRC,GP180,LCA,T200,CD9 partner 1,CD9P-1,Glu-Trp-Ile EWI motif-containing protein F,EWI-F,Prostaglandin F2-alpha receptor regulatory protein,Prostaglandin F2-alpha receptor-associated protein,Protein-tyrosine phosphatase eta,R-PTP-eta,Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen (L-CA),Lymphocyte antigen 5 (Ly-5),Ptprc,Ly-5
货号： S0B5035
- 价格： ￥650
- 规格：
- 数量：
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宿主来源
Rat抗原名称
CD45R/B220分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; Ptprc; Ly-5细胞定位
Cell membraneAccession
P06800克隆号
S-R519抗体类型
Rat mAb抗体同种型
IgG2a,k应用
FCM反应种属 ?
Hu, Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Hu, Ms |
CD45R, also known as B220, is a protein tyrosine phosphatase that is predominantly found on B cells, at varying levels from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. CD45R plays a crucial role in B cell receptor and T cell receptor signaling and is essential for lymphocyte development and function. It helps regulate the threshold of sensitivity to external stimuli, affecting cellular responses. In mice, CD45R/B220 is a common marker for B cells, whereas in humans, B cells do not express B220 in the same way; it appears to be differentiation-specific and is therefore expressed on only some B cell subsets. CD45R is also used in the classification of leukemias and lymphomas and in research on functional subsets of B and T cells.
流式分析
Flow cytometric analysis of CD45R/B220 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either FITC Rat IgG2a, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse/Human CD45R/B220 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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