FITC Mouse Anti-Mouse CD45.1 Antibody (S-R521)
CD45,PTPRC,GP180,LCA,T200,CD9 partner 1,CD9P-1,Glu-Trp-Ile EWI motif-containing protein F,EWI-F,Prostaglandin F2-alpha receptor regulatory protein,Prostaglandin F2-alpha receptor-associated protein,Protein-tyrosine phosphatase eta,R-PTP-eta,Ly5.1
货号： S0B5032
- 价格： ￥650
- 规格：
- 数量：
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宿主来源
Mouse抗原名称
Mouse CD45.1分子别名
Ly5.1细胞定位
Cell membraneAccession
P06800克隆号
S-R521抗体类型
Mouse mAb抗体同种型
IgG2a,k应用
FCM反应种属 ?
Ms阳性样本
SJL mouse splenocytes纯化方式
Protein A浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5μl per million cells in 100μl volume | Ms |
CD45.1 is a protein isoform of CD45, also known as PTPRC, which is a receptor-type tyrosine phosphatase expressed on all hematopoietic cells except erythrocytes and platelets. This type I transmembrane protein plays a crucial role in the activation processes of B and T cell receptors, as well as in thymic selection. CD45.1, along with CD45.2, is commonly used in adoptive cell transfer experiments to distinguish donor cells from host cells due to their allelic differences. Mice carrying the CD45.1 allele, such as C57BL/6J, are widely used in research for this purpose. CD45.1 is also used in studies to understand the rejuvenating effects of young blood on aged tissues. In heterochronic parabiosis models, where the circulatory systems of old and young mice are connected, CD45.1 and CD45.2 genotypes are used to track the cells of old and young mice, respectively.
流式分析
Flow cytometric analysis of Mouse CD45.1 expression on mouse splenocytes. Mouse splenocytes from a C57BL/6 mouse (Left Panel) and SJL mouse (Right Panel) were separately stained with FITC Mouse IgG2a, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Mouse CD45.1 Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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