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产品规格
宿主来源
Mouse抗原名称
CD8分子别名
T-lymphocyte differentiation antigen T8/Leu-2; T-cell surface glycoprotein CD8 alpha chain; MAL; CD8a细胞定位
Cell membraneAccession
P01732克隆号
S-R533抗体类型
Mouse mAb抗体同种型
IgG1应用
FCM反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
稀释度
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1.25μl per million cells in 100μl volume | Hu |
背景介绍
CD8A encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
流式分析
Flow cytometric analysis of Human CD8 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either FITC Mouse IgG1 Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD8 Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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