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宿主来源
Goat抗原名称
Rabbit IgG抗体类型
Polyclonal antibody反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
2 mg/ml性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICC ?
IF ?
稀释度
应用 稀释度 推荐种属 ICC 1:1000 Hu IF 1:1000 Hu
免疫细胞化学
ICC shows positive staining in 293T cells. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C2C12 cells. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human endometrial cancer. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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