分享产品介绍 评论(0)
宿主来源
Goat抗原名称
Mouse IgG抗体类型
Polyclonal antibody反应种属 ?
Ms纯化方式
Immunogen Affinity浓度
2 mg/ml标记
TRITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azid
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:1000 Ms
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti- α-tubulin antibody (S0B0645) was used at 1/1000 dilution (Red) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (TRITC Conjugate) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti-α-tubulin antibody (S0B0645) was used at 1/1000 dilution (Red) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (TRITC Conjugate) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
评论(0)