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抗体类型
Polyclonal antibody反应种属 ?
Rt纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 594性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICC ?
IF ?
稀释度
应用 稀释度 推荐种属 ICC 1:1000 Rt IF 1:1000 Rt
免疫荧光
IF shows positive staining in paraffin-embedded human testis. Anti-EWSR1/EWS antibody (S0B0280) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse testis. Anti-EWSR1/EWS antibody (S0B0280) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti-EWSR1/EWS antibody (S0B0280) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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