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宿主来源
Rabbit抗原名称
Mouse IgG免疫原
Native protein抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms纯化方式
Protein A浓度
5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
ELISA
WB
稀释度
应用 稀释度 推荐种属 ELISA 1:5000-1:10000 Ms WB 1:5000-1:10000 Ms FCM 1:500 Ms
免疫印迹
WB result of Rabbit Anti-Mouse IgG (H+L) mAb
Primary antibody: Rabbit Anti-Mouse IgG (H+L) mAb at 1/5000 dilution
Lane 1: mouse serum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25, 55 kDa
Observed MW: 26, 50 kDa
流式分析
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / U266B1 (Human multiple myeloma B lymphocyte, right) labeled with SDT Mouse Anti-Human CD70 antibody at 1/200 dilution (1 μg) (S0B5570) followed by Rabbit Anti-Mouse IgG (H+L) mAb at 1/500 dilution (1 μg). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the tertiary antibody.Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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