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宿主来源
Goat抗原名称
Rabbit IgG免疫原
Rabbit IgG抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
1.5 mg/ml标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ELISA
WB
稀释度
应用 稀释度 推荐种属 ELISA 1:1000-1:3000 Rb WB 1:5000-1:10000 Rb
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment is a type of secondary antibody fragment that is widely utilized in various immunological assays due to its specific characteristics. Derived from immunoglobulins of goats that have been immunized against rabbit IgG, this fragment consists of the antigen-binding regions (Fab')2 of the antibody molecule, which includes both the heavy (H) and light (L) chains. The F(ab')2 fragment is obtained by enzymatic digestion of the whole IgG molecule, specifically using pepsin, which cleaves the molecule into pieces while retaining the antigen-binding sites.
免疫印迹
WB result of Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (HRP Conjugate)
Primary antibody: β-actin Rabbit mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (HRP Conjugate) at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
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