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宿主来源
Goat抗原名称
Rat IgG抗体类型
Polyclonal antibody反应种属 ?
Rt纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
ICC ?
IF ?
稀释度
应用 稀释度 推荐种属 ICC 1:2000 Rt IF 1:2000 Rt
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/2000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded mouse testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti- RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/2000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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