大包装询价 产品介绍 评论(0)
宿主来源
Goat抗原名称
Rabbit IgG免疫原
Rabbit IgG抗体类型
Polyclonal antibody应用
ICC, IF反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Rb |
| IF | 1:500 | Rb |
免疫细胞化学
ICC shows positive staining in MCF7 cells. Anti-Ki67 antibody (S0B2332) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (FITC conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-Ki67 antibody (S0B2332) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (FITC conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
评论(0)