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宿主来源
Goat抗原名称
Rat IgG免疫原
Rabbit IgG抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Rt纯化方式
Immunogen Affinity浓度
2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
IF ?
稀释度
应用 稀释度 推荐种属 IF 1:1000-1:2000 Rt
免疫荧光
IF shows positive staining in paraffin-embedded mouse testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (FITC Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat testis. Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (S0B0284) was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (FITC Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.







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