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宿主来源
Goat抗原名称
Rabbit IgG免疫原
Rabbit IgG抗体类型
Polyclonal antibody反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
2 mg/ml标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.
应用
IHC-P ? ,ELISA ,WB稀释度
应用 稀释度 WB 1:10000 IHC-P 1:100-1:500 ELISA 1:10000
This antibody has been tested by ELISA to ensure minimal cross-reaction with human serum proteins.
免疫印迹
WB result of Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot), HRP
Primary antibody: Kappa light chain (S0B0083) Rabbit mAb at 1/1000 dilution
Lane 1: human serum lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PAX8 antibody (S0B2071) was used at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot), HRP at 1:100 dilution. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-PAX8 antibody (S0B2071) was used at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot), HRP at 1:100 dilution. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-EpCAM antibody (S0B2028) was used at 1/500 dilution, followed by Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot), HRP at 1:500 dilution. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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