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宿主来源
Goat抗体类型
Polyclonal antibody反应种属 ?
Ms纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
ICC ?
IF ?
稀释度
应用 稀释度 ICC 1:500 IF 1:500
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin antibody (S0B0102) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat anti-Mouse IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-PD-1 antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4008) was used as secondary antibody at 1/500 dilution.Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human liver. Anti-PD-1 antibody was used at 1/200 dilution and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 647 Conjugate) (S0B4008) was used as secondary antibody at 1/500 dilution.Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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