Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)
Rabbit IgG,Rabbit Immunoglobulin G,Rabbit IgG Heavy Chain,Rabbit IgG γ,Rabbit IgGγ,Rabbit IgG gamma,Rabbit IgG Light Chain,Rabbit IgG κ,Rabbit IgGκ,Rabbit IgG kappa,Rabbit IgG λ,Rabbit IgGλ,Rabbit IgG lambda,Rabbit IgG Fc,Rabbit IgG Fab,Rabbit IgG (H+L),rabbit IgG
货号： S0B4007
- 价格： ￥120
- 规格：
- 数量：
大包装询价 产品介绍 引用文献(1) 评论(0)
宿主来源
Goat抗原名称
Rabbit IgG免疫原
Rabbit IgG抗体类型
Polyclonal antibody应用
ICC, IF反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 594性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| ICC | 1:500 |
| IF | 1:500 |
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in MCF7 cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in MCF7 cells. Anti-α-tubulin antibody (S0B0043) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human colon cancer. Anti-CK-HMW antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate) (S0B4007) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human thyoid follicular carcinoma. Anti-CK-HMW antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate) (S0B4007) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
引用文献(1)
- Modular Design of Lipopeptide‐Based Organ‐Specific Targeting (POST) Lipid Nanoparticles for Highly Efficient RNA Delivery
C Tang, Y Zhang, B Li, X Fan, Z Wang, R Su
advanced materials. 2025 Feb 09 ; 63
影响因子： 25.8
货号：S0B4007产品名称：Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)
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