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Goat anti-Rabbit IgG(H+L) (Alexa Fluor^® 594 Conjugate)

浏览次数：43

货号： S0B4007

Datasheet COA

价格： ￥120
规格：
25μl100μl1ml
数量：

大包装询价

产品介绍引用文献(1) 评论(0)

产品规格

宿主来源

Goat
抗原名称

Rabbit IgG
免疫原

Rabbit IgG
抗体类型

Polyclonal antibody
应用

ICC, IF
反应种属 ?

Rb
纯化方式

Immunogen Affinity
浓度

2 mg/ml
标记

Alexa Fluor® 594
性状

Liquid
缓冲体系

PBS, 1% BSA, 0.3% Proclin 300
储存条件

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

稀释度

应用	稀释度
ICC	1:500
IF	1:500

免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in MCF7 cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in MCF7 cells. Anti-α-tubulin antibody (S0B0043) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
- IF shows positive staining in paraffin-embedded human colon cancer. Anti-CK-HMW antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate) (S0B4007) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- Negative control: IF shows negative staining in paraffin-embedded human thyoid follicular carcinoma. Anti-CK-HMW antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate) (S0B4007) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

引用文献(1)

Modular Design of Lipopeptide‐Based Organ‐Specific Targeting (POST) Lipid Nanoparticles for Highly Efficient RNA Delivery
C Tang, Y Zhang, B Li, X Fan, Z Wang, R Su

advanced materials. 2025 Feb 09 ; 63

影响因子： 25.8

货号：S0B4007产品名称：Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)