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Goat anti-Rabbit IgG (H+L) (Alexa Fluor^® 647 Conjugate)

浏览次数：79

货号： S0B4005

Datasheet COA

价格： ￥120
规格：
25μl100 μl1ml
数量：

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

宿主来源

Goat
抗原名称

Rabbit IgG
分子别名

N/A
免疫原

Rabbit IgG
细胞定位

N/A
克隆号

N/A
抗体类型

Polyclonal antibody
应用

ICC, IF
反应种属 ?

Rb
纯化方式

Immunogen Affinity
浓度

2 mg/ml
标记

Alexa Fluor® 647
性状

Liquid
缓冲体系

PBS, 0.1% BSA, 0.01% Proclin 300
储存条件

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

稀释度

应用	稀释度
ICC	1:500
IF	1:500

背景介绍

免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
- IF shows positive staining in paraffin-embedded human tonsil. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human prostate. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.