Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate)
Rabbit IgG,Rabbit Immunoglobulin G,Rabbit IgG Heavy Chain,Rabbit IgG γ,Rabbit IgGγ,Rabbit IgG gamma,Rabbit IgG Light Chain,Rabbit IgG κ,Rabbit IgGκ,Rabbit IgG kappa,Rabbit IgG λ,Rabbit IgGλ,Rabbit IgG lambda,Rabbit IgG Fc,Rabbit IgG Fab,Rabbit IgG (H+L),rabbit IgG,N/A
货号： S0B4005
- 价格： ￥120
- 规格：
- 数量：
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宿主来源
Goat抗原名称
Rabbit IgG分子别名
N/A免疫原
Rabbit IgG细胞定位
N/A克隆号
N/A抗体类型
Polyclonal antibody应用
ICC, IF反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 0.1% BSA, 0.01% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| ICC | 1:500 |
| IF | 1:500 |
N/A
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostate. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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