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Goat抗原名称
Rabbit IgG分子别名
N/A免疫原
Rabbit IgG细胞定位
N/A克隆号
N/A抗体类型
Polyclonal antibody反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
ICC ? ,IF ?稀释度
应用 稀释度 ICC 1:500 IF 1:500
N/A
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostate. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 647 Conjugate) (S0B4005) was used at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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