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Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) STAR

N/A

价格 ￥270.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B4004
规格
数量
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产品介绍 引用文献(3) 评论(0)

产品规格

  • 宿主来源

    Goat

  • 抗原名称

    Rabbit IgG

  • 分子别名

    N/A

  • 免疫原

    Rabbit IgG

  • 细胞定位

    N/A

  • 克隆号

    N/A

  • 抗体类型

    Recombinant mAb

  • 应用

    FCM, ICC, IF

  • 反应种属 ?

    Rb

  • 纯化方式

    Immunogen Affinity

  • 浓度

    Lot specific* (generally 1.5 to 2 mg/ml)*

  • 标记

    Alexa Fluor® 488

  • 性状

    Liquid

  • 缓冲体系

    PBS, 0.1% BSA, 0.01% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

稀释度
应用 稀释度
ICC 1:1000-1:2000
FCM 1:2000
IF 1:1000-1:2000
背景介绍

  • N/A

  • 流式分析

    • Flow cytometric analysis of mouse splenocytes labeling CD8a antibody (S0B0034) at 1/50 dilution (1 μg) (Right) compared with a Rabbit monoclonal IgG isotype control (Left). Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as the secondary antibody at 1/2000 dilution.

      Cells were surface stained with CD4-Alexa Fluor® 647, then stained with rabbit IgG (Left) / anti-CD8a (Right) separately. CD8a and CD4 are mutually exclusive expressed in mouse splenocytes. Gated on total viable cells.

    • Flow cytometric analysis of HCT 116 cells labelling CD9 antibody (S0B0027) at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as the secondary antibody at 1/2000 dilution.

    • Flow cytometric analysis of A431 cells labelling Laminin gamma 1 antibody (S0B0069) at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as the secondary antibody at 1/2000 dilution.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human colon cancer. Anti-CK-LMW antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) (S0B4004) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human thyroid cancer. Anti-CK-LMW antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) (S0B4004) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded human keratinized oral squamous cell carcinoma. Anti-CK-LMW antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) (S0B4004) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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