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宿主来源
Goat抗原名称
Rabbit IgG免疫原
Rabbit IgG克隆号
No clone Number抗体类型
Goat pAb应用
ELISA, WB反应种属 ?
Rb纯化方式
Immunogen Affinity浓度
Lot specific* (generally 1.5 to 2.2 mg/ml)*标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| ELISA | 1:5000-1:30000 |
| WB | 1:5000-1:20000 |
OneStep polymer HRP is the enzyme polymer conjugated with secondary antibodies. The labeled polymer does not contain avidin or biotin, thus, non-specific staining caused by endogenous biotin is completely eliminated. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. The secondary antibody is intended for use with primary antibodies from rabbit for localization and identification of specific antigens on a slide mounted tissue sections by light microscopy.
验证数据
S0B4002 or secondary antibody from competitor was used at 1/10000 dilution in ELISA
Recommend Dilution:1:5000-1:30000
免疫印迹
Primary antibody :β-Tubulin (S0B0004) Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µgSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP (S0B4002)
Molecular weight: 50 kDaRecommend Dilution:1:5000-1:20000
Primary antibody :β-Tubulin (S0B0004) Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µgSecondary antibody: Goat anti-Rabbit IgG HRP from conpetitor at 1/10000 dilution
Molecular weight: 50 kDaRecommend Dilution:1:5000-1:20000
引用文献(2)
- Induction of hepatic fibrosis in mice with schistosomiasis by extracellular microRNA-30 derived from Schistosoma japonicum eggs
Y Chen, Y Hu, H Zhou, N Jiang, Y Wang
Frontiers in Immunology. 2024 Jul 30 .
影响因子： 5.7
货号：S0B4002产品名称：Goat anti-Rabbit IgG(H+L), HRP
- Intracellular metabotropic glutamate receptor 5 in spinal dorsal horn neurons contributes to pain in a mouse model of vincristine-induced neuropathic pain
X Li, H Yang, M Qian, H Liu, S Zuo, JC Liu, WH Ge
Neuroscience Letters. 2025 Apr 16 .
影响因子： 2.5
货号：S0B4002产品名称：Goat anti-Rabbit IgG(H+L), HRP
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