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宿主来源
Goat克隆号
No clone Number,IgG(H+L)抗体类型
Goat pAb反应种属 ?
Rb标记
HRP性状
Liquid储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
IHC-P ?
OneStep polymer HRP is the enzyme polymer conjugated with secondary antibodies. The labeled polymer does not contain avidin or biotin, thus, non-specific staining caused by endogenous biotin is completely eliminated. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. The secondary antibody is intended for use with primary antibodies from rabbit for localization and identification of specific antigens on a slide mounted tissue sections by light microscopy.
免疫组化
IHC shows positive staining in paraffin-embedded stromal of human breast cancer.
Anti-Vimentin antibody(S0B2004) was used at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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