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宿主来源
Rabbit抗原名称
25-OH VD3分子别名
Calcifediol, 25-Hydroxy Vitamin D3, 25-Hydroxycholecalciferol克隆号
SDT-321-345抗体类型
Recombinant mAb抗体同种型
IgG交叉反应
It also reacts with 25-OH-VD2
纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt, 2 to 8 °C as supplied
应用
Competitive ELISA
25-hydroxyvitamin D3 (25-OH D3) is the major circulating form of vitamin D and the preferred biomarker for assessing overall vitamin D status. It is measured in serum or plasma, typically via immunoassay or liquid chromatography-tandem mass spectrometry (LC-MS/MS). This diagnostic test quantifies the body's vitamin D reserves, reflecting both cutaneous synthesis and dietary intake. It is crucial for evaluating deficiency, insufficiency, sufficiency, or toxicity. Results guide clinical decisions in managing bone health (e.g., osteoporosis, rickets), and are increasingly used in research linking vitamin D to immune, cardiovascular, and metabolic disorders. Consistent and accurate measurement remains essential for reliable diagnosis and therapeutic monitoring.
竞争ELISA
Competitive ELISA binding of 25-OH-VD3 recombinant mAb (S0B3602) with DNP-BSA conjugate.
Free 25-OH VD3 blocks 25-OH-VD3 recombinant mAb (S0B3602) binding with coating antigen (VD3-BSA conjugate)
Coating antigen: VD3-BSA conjugate, 0.5 µg/ml
S0B3603: 400 ng/ml
Free 25-OH-VD3 dilutions start from 200 ng/ml.
IC50= 4.043 ng/ml.







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