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宿主来源
Rabbit抗原名称
Cyclin E1分子别名
G1/S-specific cyclin-E1; CCNE; CCNE1免疫原
Synthetic Peptide细胞定位
NucleusAccession
P24864克隆号
SDT-4375-44抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MCF7, HepG2, HT-29, PC-3, HCT 116, OVCAR3, Jurkat, HeLa, JAR, K-562纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
Cyclin E1 is a regulatory subunit of the cyclin-dependent kinase Cdk2 that drives the G1-S phase transition by phosphorylating substrates such as the retinoblastoma protein, thereby releasing E2F transcription factors and committing cells to DNA replication; its expression peaks in late G1, is tightly controlled by ubiquitin-mediated proteolysis, and is frequently amplified or overexpressed in cancers, leading to genomic instability and aggressive tumor behavior.
免疫印迹
WB result of Cyclin E1 Recombinant Rabbit mAb
Primary antibody: Cyclin E1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Lane 6: OVCAR3 whole cell lysate 20 µg
Lane 7: Jurkat whole cell lysate 20 µg
Lane 8: HeLa whole cell lysate 20 µg
Lane 9: JAR whole cell lysate 20 µg
Lane 10: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 38-47 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Cyclin E1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Cyclin E1 in tumor tissues.
Expression of Cyclin E1 in human tissues.
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