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宿主来源
Rabbit抗原名称
CDH17分子别名
Cadherin-17; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin (LI-cadherin)免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q12864克隆号
SDT-3276-83抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
LoVo, Caco-2纯化方式
Protein A浓度
1mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu
CDH17, also known as liver-intestine cadherin (LI-cadherin), is a unique calcium-dependent transmembrane glycoprotein primarily and specifically expressed in the epithelial cells of the gastrointestinal tract and pancreatic ducts. Structurally, its extracellular region contains seven cadherin repeat domains (EC1-EC7), but its cytoplasmic tail is extremely short, consisting of only about 20 amino acids, preventing it from binding to α/β-catenin like classical cadherins. Functionally, CDH17 plays a dual role: it acts as a homophilic cell adhesion molecule maintaining the structural integrity of the intestinal epithelium, and also functions as a proton-dependent peptide transporter involved in the absorption of oral peptide drugs. Furthermore, in various gastrointestinal cancers (such as colorectal cancer, gastric cancer, and hepatocellular carcinoma), CDH17 is frequently overexpressed and promotes tumor cell proliferation, invasion, and metastasis by activating signaling pathways such as Wnt/β-catenin and MAPK. Consequently, it is regarded as an important cancer diagnostic biomarker and a highly promising therapeutic target. Currently, bispecific antibodies and CAR-T cell therapies targeting CDH17 have demonstrated significant anti-tumor effects in preclinical studies.
免疫印迹
WB result of CDH17 Recombinant Rabbit mAb
Primary antibody: CDH17 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: LoVo whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 130 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Caco-2 cells (top panel) and negative staining in PANC-1 cells (below panel). Anti- CDH17 antibody was used at 1/××× dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CDH17 in tumor tissues.
Expression of CDH17 in human tissues.
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