CEA Recombinant Rabbit mAb (SDT-098-41)
Cell adhesion molecule CEACAM5,Carcinoembryonic antigen (CEA),Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA cell adhesion molecule 5Imported),Meconium antigen 100,CD66e,CEA s,CEACAM5
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宿主来源
Rabbit抗原名称
CEA分子别名
Cell adhesion molecule CEACAM5; Carcinoembryonic antigen (CEA); Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA cell adhesion molecule 5Imported); Meconium antigen 100; CD66e; CEA s; CEACAM5免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P06731克隆号
SDT-098-41抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MCF7, HT-29, BxPC-3纯化方式
Protein A浓度
0.1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:1000-1:2000 Hu
CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5), commonly known as CEA (Carcinoembryonic Antigen), is an acidic glycoprotein widely expressed in human epithelial tissues and belongs to the immunoglobulin superfamily. Structurally, it is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) moiety and lacks an intracellular signal transduction domain. Under physiological conditions, CEACAM5 is highly expressed only in the gastrointestinal tract, pancreas, and liver during early fetal development, with expression levels significantly decreasing after birth. However, under pathological conditions, particularly in various malignant tumors such as colorectal cancer, gastric cancer, lung cancer, pancreatic cancer, and breast cancer, CEACAM5 is frequently overexpressed. As a classic tumor marker, serum CEACAM5 levels have been widely used for cancer clinical diagnosis, treatment efficacy monitoring, and recurrence prediction. Functionally, as a cell adhesion molecule, CEACAM5 primarily regulates tumor cell aggregation, polarity, migration, and invasion through homophilic or heterophilic interactions, while also inhibiting differentiation and blocking anoikis, thereby promoting tumor metastasis and drug resistance. Due to its highly specific expression in tumor tissues, CEACAM5 has become an important target for antibody-based targeted therapy and immunotherapy.
免疫印迹
WB result of CEA Recombinant Rabbit mAb
Primary antibody: CEA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: BxPC-3 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 150-300 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CEA antibody was used at 1/1200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CEA antibody was used at 1/1200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CEA antibody was used at 1/1200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-CEA antibody was used at 1/1200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CEA in human tissues.
Expression of CEA in tumor tissues.
Expression of CEA in colon cancer tissues.
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