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宿主来源
Rabbit抗原名称
FOLR1分子别名
Folate receptor alpha; FR-alpha; Adult folate-binding protein (FBP); Folate receptor 1; Folate receptor; adult; KB cells FBP; Ovarian tumor-associated antigen MOv18; FOLR免疫原
Recombinant Protein细胞定位
Secreted, Endosome, Cell membraneAccession
P15328克隆号
SDT-3635-124抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
JAR, HeLa, SK-OV-3, OVCAR-3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:500 Hu, Ms, Rt
Human folate receptor alpha (FOLR1) is a glycoprotein that plays a crucial role in the transport of folate, an essential vitamin, into cells. This receptor is highly expressed in certain tissues and is particularly notable for its involvement in various physiological processes including embryonic development and cellular proliferation. Its overexpression has been observed in numerous cancers, such as ovarian, lung, and breast cancer, making it a potential target for cancer diagnosis and targeted therapy. By binding to folate or folate analogs, FOLR1 facilitates the internalization of these molecules via endocytosis, thereby providing cells with the necessary folate for metabolic functions like DNA synthesis and repair.
免疫印迹
WB result of FOLR1 Recombinant Rabbit mAb
Primary antibody: FOLR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: JAR whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Lane 5: OVCAR-3 whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 36 kDaWB result of FOLR1 Recombinant Rabbit mAb
Primary antibody: FOLR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 35 kDaWB result of FOLR1 Recombinant Rabbit mAb
Primary antibody: FOLR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 32 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer (case 1). Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer (case 2). Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-FOLR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FOLR1 in tumor tissues.
Expression of FOLR1 in ovarian cancer tissues.
Expression of FOLR1 in human tissues.
Expression of FOLR1 in mouse & rat tissues.
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