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ABCC1/MRP1 Recombinant Rabbit mAb (SDT-4056-11)

Multidrug resistance-associated protein 1,ATP-binding cassette sub-family C member 1,Glutathione-S-conjugate-translocating ATPase ABCC1,Leukotriene C(4) transporter (LTC4 transporter),MRP

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2483
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ABCC1

  • 分子别名

    Multidrug resistance-associated protein 1; ATP-binding cassette sub-family C member 1; Glutathione-S-conjugate-translocating ATPase ABCC1; Leukotriene C(4) transporter (LTC4 transporter); MRP

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cell membrane

  • Accession

    P33527

  • 克隆号

    SDT-4056-11

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Mk

  • 阳性样本

    SK-MEL-2, HepG2, PANC-1, A549, HeLa, BxPC-3, SK-OV-3, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Mk
    IHC-P 1:1000 Hu
背景介绍

  • ABCC1, also known as multidrug resistance-associated protein 1 (MRP1), is a 180–190 kDa integral membrane protein that belongs to the ATP-binding cassette (ABC) transporter superfamily. It contains two membrane-spanning domains, each with six α-helices, and two nucleotide binding domains, along with an additional third membrane-spanning domain. This protein functions as a multispecific organic anion transporter, with substrates including oxidized glutathione, cysteinyl leukotrienes, activated aflatoxin B1, glucuronides, and sulfate conjugates of steroid hormones and bile salts. It is highly conserved and expressed in various tissues, particularly in the lungs, spleen, testes, kidneys, placenta, thyroid, bladder, adrenal glands, and endothelium cells of the blood-brain barrier. ABCC1 plays a significant role in multidrug resistance of cancerous tumor cells by transporting chemotherapeutic drugs out of the cells. Additionally, it has been a point of interest in Alzheimer's disease research due to its ability to decrease β-amyloid accumulation in the brain.

  • 免疫印迹

    • WB result of ABCC1/MRP1 Recombinant Rabbit mAb
      Primary antibody: ABCC1/MRP1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled SK-MEL-2 whole cell lysate 20 µg
      Lane 2: unboiled HepG2 whole cell lysate 20 µg
      Lane 3: unboiled PANC-1 whole cell lysate 20 µg
      Lane 4: unboiled A549 whole cell lysate 20 µg
      Lane 5: unboiled HeLa whole cell lysate 20 µg
      Lane 6: unboiled BxPC-3 whole cell lysate 20 µg
      Lane 7: unboiled SK-OV-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 171 kDa
      Observed MW: 180-300 kDa

    • WB result of ABCC1/MRP1 Recombinant Rabbit mAb
      Primary antibody: ABCC1/MRP1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 171 kDa
      Observed MW: 180-300 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreas cancer. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human Squamous Cell Lung Cancer. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of ABCC1/MRP1 in tumor tissues.

    • Expression of ABCC1/MRP1 in human tissues.

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