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宿主来源
Rabbit抗原名称
NeuroD1分子别名
Neurogenic differentiation factor 1; NeuroD; NeuroD1Alternative nameClass A basic helix-loop-helix protein 3 (bHLHa3); BHLHA3; NEUROD1免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q13562克隆号
SDT-3843-59抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Y79, β-TC-6, mouse cerebellum, rat cerebellum纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu IF 1:100 Ms, Rt
NeuroD1 (Neurogenic Differentiation 1) is a basic helix-loop-helix (bHLH) transcription factor that serves as a master regulator in the development of the nervous system and pancreas, primarily functioning to commit progenitor cells to a neuronal fate by activating downstream neurogenic genes while simultaneously suppressing glial differentiation pathways. Expressed transiently during embryogenesis in specific regions of the central and peripheral nervous systems, NeuroD1 binds to E-box DNA sequences as a heterodimer with E-proteins to drive the expression of targets essential for neuronal maturation, migration, and survival, making it critical for the formation of structures such as the hippocampus, cerebellum, and retina; beyond its developmental role, it is also pivotal in pancreatic beta-cell differentiation and has been identified as a potent reprogramming factor capable of directly converting non-neuronal cells, including astrocytes and fibroblasts, into functional neurons, thereby holding significant therapeutic potential for treating neurodegenerative diseases and repairing neural injury through endogenous regeneration or cell replacement strategies.
免疫印迹
WB result of NeuroD1 Recombinant Rabbit mAb
Primary antibody: NeuroD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Y79 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDaWB result of NeuroD1 Recombinant Rabbit mAb
Primary antibody: NeuroD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: β-TC-6 whole cell lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDaWB result of NeuroD1 Recombinant Rabbit mAb
Primary antibody: NeuroD1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human pancreas. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse retina. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-NeuroD1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Y79 cells (top panel) and negative staining in HeLa cells (below panel). Anti-NeuroD1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebellum. Anti- NeuroD1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebellum. Anti- NeuroD1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of NeuroD1 in tumor tissues.
Expression of NeuroD1 in human tissues.
Expression of NeuroD1 in mouse & rat tissues.
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