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宿主来源
Mouse抗原名称
CDKN2A/p16 INK4A分子别名
Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A (CDK4I); Multiple tumor suppressor 1 (MTS-1); p16-INK4a (p16-INK4; p16INK4A); CDKN2A; CDKN2; MTS1细胞定位
Nucleus, MitochondrionAccession
P42771克隆号
SDT-4677抗体类型
Mouse mAb反应种属 ?
Hu阳性样本
HeLa, HEK-293, Saos-2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:250-1:500 Hu IHC-P 1:1000 Hu ICC 1:500 Hu
CDKN2A/p16INK4A is a critical tumor suppressor protein encoded by the CDKN2A gene that functions as a key negative regulator of the cell cycle by specifically inhibiting cyclin-dependent kinases 4 and 6 (CDK4/6), thereby preventing the phosphorylation of the retinoblastoma protein (Rb) and arresting cell progression from the G1 to the S phase to curb uncontrolled proliferation. Often referred to as p16, this protein acts as a vital checkpoint against oncogenic transformation, and its loss of function through genetic deletion, mutation, or epigenetic silencing is a hallmark event in numerous human cancers, including melanoma, pancreatic ductal adenocarcinoma, and glioblastoma; conversely, its overexpression is frequently observed in cells undergoing senescence or in HPV-associated malignancies where it serves as a reliable diagnostic biomarker due to the disruption of the Rb pathway by viral oncoproteins, making p16INK4A not only a fundamental component of cellular defense mechanisms but also a significant target for therapeutic strategies and prognostic assessments in oncology.
免疫印迹
WB result of CDKN2A/p16 INK4A Recombinant Rabbit mAb
Primary antibody: CDKN2A/p16 INK4A Recombinant Rabbit mAb at 1/250 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: Saos-2 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervix. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human bladder cancer. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells (top panel) and negative staining in MCF7 cells (below panel). Anti-CDKN2A/p16 INK4A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CDKN2A/p16 INK4A in tumor tissues.
Expression of CDKN2A/p16 INK4A in human tissues.
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