分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
TBR2/Eomes分子别名
Eomesodermin homolog; T-box brain protein 2 (T-brain-2; TBR-2); EOMES免疫原
Synthetic Peptide细胞定位
NucleusAccession
O95936克隆号
SDT-1101-231抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
NK-92, mouse E14 embryo brain, rat E14 embryo brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt IF 1:500 Ms, Rt
TBR2/Eomes is a T-box transcription factor that plays crucial roles in various developmental processes. It is preferentially enriched in intermediate progenitors and supports cortical neurogenesis expansion. In the developing cerebral cortex, it is specifically expressed in intermediate progenitors (IPs), which are derived from radial glia progenitors and generate glutamatergic projection neurons. TBR2/Eomes is essential for the differentiation and subtype specification of these neurons, and it regulates the transcriptome by activating or repressing hundreds of direct target genes. Additionally, TBR2/Eomes is involved in the formation and maintenance of certain retinal ganglion cells. It also plays a role in the maintenance of the trophectoderm lineage by sustaining chromatin accessibility and promoting gene regulatory networks.
免疫印迹
WB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NK-92 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 55-85 kDaWB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse E14 embryo brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 60- 90 kDaWB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat E14 embryo brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 80, 90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-TBR2/Eomes antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse E14 embryo. Anti-TBR2/Eomes antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat E14 embryo. Anti-TBR2/Eomes antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of TBR2/Eomes in tumor tissues.
Expression of TBR2/Eomes in human tissues.
Expression of TBR2/Eomes in mouse & rat tissues.
评论(0)