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Occludin Recombinant Rabbit mAb,PBS Only (SDT-4588)

OCLN

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货号 S0B2475P
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Occludin

  • 分子别名

    OCLN

  • 细胞定位

    Cell membrane

  • Accession

    Q16625

  • 克隆号

    SDT-4588

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    Caco-2, HepG2, A431, A549, HT-29, bEnd.3, mouse colon, mouse small intestine, mouse liver, mouse kidney, mouse brain, mouse lung, rat colon, rat liver, rat kidney, rat brain, rat lung

  • 预测反应种属
    (反应种属缩写表)

    Dg

  • 纯化方式

    Protein A

  • 浓度

    1 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS

  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:100 Hu
背景介绍

  • Occludin is a approximately 65 kDa four-pass transmembrane protein, identified and named in 1993 as a pivotal component of tight junctions (TJs), primarily localized at the apical lateral membranes of epithelial and endothelial cells; featuring two extracellular loops, one intracellular loop, and a long C-terminal cytoplasmic tail, its extracellular loops mediate "zipper-like" sealing between adjacent cells via homo- or heterophilic interactions, thereby establishing a selective permeability barrier that strictly regulates paracellular transport and maintains cell polarity, while its C-terminus anchors tight junctions to the actin cytoskeleton by binding to cytoplasmic scaffolding proteins such as ZO-1, subsequently participating in the fine-tuned regulation of cell signaling, gene expression, proliferation, and differentiation, playing an indispensable central role in the construction of the blood-brain barrier, maintenance of tissue homeostasis, and pathological processes including inflammation and tumor metastasis.

  • 免疫印迹

    • WB result of Occludin Recombinant Rabbit mAb
      Primary antibody: Occludin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Caco-2 whole cell lysate 20 µg
      Lane 3: HepG2 whole cell lysate 20 µg
      Lane 4: A431 whole cell lysate 20 µg
      Lane 5: A549 whole cell lysate 20 µg
      Lane 6: HT-29 whole cell lysate 20 µg
      Low expression control: HeLa whole cell lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 18-60 kDa

    • WB result of Occludin Recombinant Rabbit mAb
      Primary antibody: Occludin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: bEnd.3 whole cell lysate 20 µg
      Lane 2: mouse colon lysate 20 µg
      Lane 3: mouse small intestine lysate 20 µg
      Lane 4: mouse liver lysate 20 µg
      Lane 5: mouse kidney lysate 20 µg
      Lane 6: mouse brain lysate 20 µg
      Lane 7: mouse lung lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 18-60 kDa

    • WB result of Occludin Recombinant Rabbit mAb
      Primary antibody: Occludin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat colon lysate 20 µg
      Lane 2: rat liver lysate 20 µg
      Lane 3: rat kidney lysate 20 µg
      Lane 4: rat brain lysate 20 µg
      Lane 5: rat lung lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 59 kDa
      Observed MW: 18-60 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-Occludin antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in Caco2 cells. Anti-Occludin antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Occludin in tumor tissues.

    • Expression of Occludin in human tissues.

    • Expression of Occludin in mouse & rat tissues.

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