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宿主来源
Rabbit抗原名称
Cytokeratin 1分子别名
Keratin, type II cytoskeletal 1; 67 kDa cytokeratin; Cytokeratin-1 (CK-1); Hair alpha protein; Keratin-1 (K1); Type-II keratin Kb1; KRTA; KRT1免疫原
Recombinant Protein细胞定位
Cytoplasm, Cell membraneAccession
P04264克隆号
SDT-3225-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
A431, HaCaT, mouse skin纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:2000-1:4000 Hu, Ms IHC-P 1:500-1: 2000 Hu, Ms, Rt IF 1:500 Hu
Cytokeratin 1 (CK1), encoded by the KRT1 gene, is a type II cytokeratin that heterodimerizes with cytokeratin 10 (CK10) to form intermediate filaments specifically expressed in the suprabasal layers of stratified squamous epithelia, including skin, oral mucosa, and esophagus. This 67-kDa protein plays a critical structural role in maintaining mechanical integrity of keratinocytes and protecting epithelial tissues from mechanical stress, osmotic shock, and apoptosis. CK1 contains a central α-helical rod domain flanked by non-helical head and tail domains, with the tail domain undergoing extensive post-translational modifications including phosphorylation and glycation that regulate filament assembly and keratinocyte differentiation. Mutations in KRT1 cause epidermolytic hyperkeratosis (EHK), a severe autosomal dominant ichthyosis characterized by epidermal blistering and hyperkeratosis due to keratin filament collapse and keratinocyte fragility. Beyond its structural functions, CK1 participates in cell signaling, wound healing, and stress response pathways, and serves as an important histopathological marker for distinguishing epithelial-derived carcinomas from mesenchymal tumors. The protein's expression pattern makes it clinically valuable for identifying squamous cell carcinomas and stratified epithelial origins in diagnostic pathology.
免疫印迹
WB result of Cytokeratin 1 Recombinant Rabbit mAb
Primary antibody: Cytokeratin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HaCaT whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 66 kDaWB result of Cytokeratin 1 Recombinant Rabbit mAb
Primary antibody: Cytokeratin 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse skin lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 66 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Cytokeratin 1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human esophageal cancer. Anti-Cytokeratin 1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human oral squamous cell carcinoma. Anti-Cytokeratin 1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Cytokeratin 1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skin. Anti-Cytokeratin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Cytokeratin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skin. Anti-Cytokeratin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Cytokeratin 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti- Cytokeratin 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Cytokeratin 1 in tumor tissues.
Expression of Cytokeratin 1 in human tissues.
Expression of Cytokeratin 1 in mouse & rat tissues.
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