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Mouse抗原名称
Napsin A分子别名
Napsin-A; Aspartyl protease 4 (ASP4; Asp 4); Napsin-1; TA01/TA02; NAP1; NAPA; NAPSA细胞定位
SecretedAccession
O96009克隆号
SDT-4204抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms, Rt纯化方式
Protein G浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, -4 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000 Hu, Ms, Rt
Napsin A is a 38-kDa, pepsin-fold aspartic protease encoded by chromosome 19q13 and expressed almost exclusively in type II pneumocytes and renal proximal tubular epithelial cells, where it participates in surfactant protein B maturation and endolysosomal protein catabolism; immunohistochemically its strong, granular cytoplasmic staining serves as a highly sensitive and specific marker for primary lung adenocarcinoma (positive in ~85 % of cases) while being consistently negative in squamous cell carcinoma, small-cell carcinoma, and most non-pulmonary adenocarcinomas except renal clear-cell carcinoma, making it a key component of modern diagnostic panels alongside TTF-1 for distinguishing pulmonary origin in metastatic settings.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Napsin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Napsin A in tumor tissue.
Expression of Napsin A in human tissue.
Expression of Napsin A in mouse & rat tissue.
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