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Rabbit抗原名称
PAM分子别名
Peptidyl-glycine alpha-amidating monooxygenase免疫原
Synthetic Peptide细胞定位
SecretedAccession
P19021克隆号
SDT-2995-30抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
NCI-H69, U-2 OS预测反应种属
(反应种属缩写表)Covering most species纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
Peptidylglycine α-amidating monooxygenase (PAM) is a copper-dependent, bifunctional enzyme that catalyzes the C-terminal amidation of peptide hormones and neuropeptides—an essential post-translational modification for their full biological activity—through two sequential catalytic domains, with its expression, localization, and trafficking tightly regulated by alternative splicing, mRNA-binding proteins, and dynamic endocytic recycling, and genetic variants in PAM have been linked to metabolic disorders such as type 2 diabetes and pituitary hormone dysfunction.
免疫印迹
WB result of PAM Recombinant Rabbit mAb
Primary antibody: PAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NCI-H69 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human medullary thyroid carcinoma. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human papillary thyroid carcinoma. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-PAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PAM in tumor tissue.
Expression of PAM in human tissue.
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