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Rabbit抗原名称
NOD2分子别名
Nucleotide-binding oligomerization domain-containing protein 2; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1; CARD15免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, MitochondrionAccession
Q9HC29克隆号
SDT-3245-112抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution,4 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Hu IF 1:100 Hu
NOD2 (nucleotide-binding oligomerization domain 2) is a 1,040-amino-acid intracellular pattern-recognition receptor that senses muramyl dipeptide from bacterial peptidoglycan through its C-terminal leucine-rich repeat domain; ligand binding relieves autoinhibition, enabling the centrally located NOD/NACHT domain to self-oligomerize, after which the dual N-terminal CARD domains recruit the adaptor kinase RIP2/RICK to activate NF-κB and MAPK cascades that drive transcription of pro-inflammatory cytokines and antimicrobial peptides, while also interacting with ATG16L1 to trigger autophagy, thereby orchestrating innate defense in macrophages, dendritic and Paneth cells—yet more than 100 germ-line mutations disrupt these processes, conferring risk for Crohn’s disease (loss-of-function LRR variants), Blau syndrome or early-onset sarcoidosis (gain-of-function NOD-domain variants) and highlighting NOD2’s critical role in balancing microbial sensing, intestinal homeostasis and autoinflammatory pathology.
免疫组化
IHC shows positive staining in paraffin-embedded human small intestine. Anti-NOD2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human placenta. Anti-NOD2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-NOD2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-NOD2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human small intestine. Anti-NOD2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of NOD2 in human tissue.
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