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宿主来源
Rabbit抗原名称
Inhibin alpha分子别名
Inhibin alpha chain; INHA免疫原
Synthetic Peptide细胞定位
SecretedAccession
P05111克隆号
SDT-3212-24抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse testis, mouse ovary, rat testis纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Ms, Rt IHC-P 1:1000 Hu, Ms, Rt IF 1:100 Hu
Inhibin alpha is a 366-residue glycoprotein that dimerizes to form the common α-subunit of the inhibin family; it is synthesized primarily in Sertoli cells of the testis and granulosa cells of the ovary, where it partners with either a βA or βB chain to create inhibin A or inhibin B, both of which act as potent negative-feedback regulators of pituitary FSH secretion by antagonizing activin signaling through competition for the type II activin receptor, thereby governing gametogenesis and steroidogenesis; beyond reproduction, inhibin alpha is a sensitive serum tumor marker for granulosa-cell and Sertoli-cell neoplasms, and its expression is controlled by gonadotropins, local growth factors, and epigenetic mechanisms, making it a key node in the hypothalamic–pituitary–gonadal axis.
免疫印迹
WB result of Inhibin alpha Recombinant Rabbit mAb
Primary antibody: Inhibin alpha Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: mouse testis lysate 20 µg
Lane 2: mouse ovary lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDaWB result of Inhibin alpha Recombinant Rabbit mAb
Primary antibody: Inhibin alpha Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 48 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian steroid cell tumor. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human seminoma. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Inhibin alpha antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human testis. Anti-Inhibin alpha chain antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human kidney. Anti-Inhibin alpha chain antibody was used at 1/200 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Inhibin alpha in tumor tissue.
Expression of Inhibin alpha in human tissue.
Expression of Inhibin alpha in mouse & rat tissue.
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