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Rabbit抗原名称
Desmoglein 3分子别名
Desmoglein-3; 130 kDa pemphigus vulgaris antigen (PVA); Cadherin family member 6; CDHF6; DSG3免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P32926克隆号
SDT-3213-33抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A431, HaCaT纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu IF 1:100 Hu
Desmoglein-3 (Dsg3) is a calcium-dependent transmembrane glycoprotein and a key component of desmosomes, specialized cell-cell adhesion structures primarily found in stratified squamous epithelia such as the skin and oral mucosa; it functions to maintain tissue integrity by anchoring intermediate filaments of the cytoskeleton to sites of intercellular contact, and beyond its structural role, Dsg3 actively regulates epithelial homeostasis by suppressing p53-mediated stress responses, thereby acting as an anti-stress protein that protects keratinocytes from apoptosis induced by UVB, mechanical stress, or genotoxic agents, while also promoting cancer cell migration and invasion through activation of the AP-1 transcription factor and PKC-dependent phosphorylation of ezrin, and its autoimmune targeting by autoantibodies results in the loss of adhesion and blister formation characteristic of pemphigus vulgaris, making Dsg3 a central player in both physiological epithelial cohesion and pathological processes including cancer metastasis and autoimmune blistering diseases.
免疫印迹
WB result of Desmoglein 3 Recombinant Rabbit mAb
Primary antibody: Desmoglein 3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A673 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HaCaT whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Low expression control: A673 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 130 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Desmoglein 3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human esophageal cancer. Anti-Desmoglein 3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Desmoglein 3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-Desmoglein 3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human esophageal cancer. Anti-Desmoglein 3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Desmoglein 3 in tumor tissue.
Expression of Desmoglein 3 in human tissue.
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