分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
INSM1分子别名
Insulinoma-associated protein 1; Zinc finger protein IA-1; IA1免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q01101克隆号
SDT-3165-72抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
NCI-H69, Y79, β-TC-6, PC-12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu
INSM1 (Insulinoma-associated 1) is a 58 kDa zinc-finger transcription factor transiently expressed during embryogenesis in neuroendocrine tissues, pancreas and nervous system, where it drives differentiation by repressing progenitor genes such as Ascl1 and NeuroD/β2 while promoting cell-cycle exit, and later in life its re-expression in neuroendocrine tumors sustains oncogenesis through feed-forward loops that stabilize MYC proteins and activate PI3K/AKT signaling.
免疫印迹
WB result of INSM1 Recombinant Rabbit mAb
Primary antibody: INSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: NCI-H69 whole cell lysate 20 µg
Lane 3: Y79 whole cell lysate 20 µg
Negative control: A549 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDaWB result of INSM1 Recombinant Rabbit mAb
Primary antibody: INSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: β-TC-6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 65 kDaWB result of INSM1 Recombinant Rabbit mAb
Primary antibody: INSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human pancreas. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-INSM1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of INSM1 in tumor tissue.
Expression of INSM1 in human tissue.
Expression of INSM1 in mouse & rat tissue.
评论(0)