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Sialoadhesin/CD169 Recombinant Rabbit mAb (SDT-3228-8)

Sialoadhesin,Sheep erythrocyte receptor (SER),Sialic acid-binding Ig-like lectin 1 (Siglec-1),Sa,Sn,Siglec1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2459
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CD169

  • 分子别名

    Sialoadhesin; Sheep erythrocyte receptor (SER); Sialic acid-binding Ig-like lectin 1 (Siglec-1); Sa; Sn; Siglec1

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cell membrane

  • Accession

    Q62230

  • 克隆号

    SDT-3228-8

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    PMA, LPS and BFA treated THP-1 cells, LPS treated RAW264.7, mouse spleen, mouse liver, rat spleen, rat liver

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
    ICC 1:500 Ms
背景介绍

  • CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the immunoglobulin superfamily expressed predominantly on subsets of tissue-resident macrophages and some dendritic cells; it contains 17 extracellular Ig-like domains that together project ~33 nm from the plasma membrane to enable high-avidity binding to α2,3-linked sialic acid moieties on glycoproteins and glycolipids of host cells or pathogens, thereby facilitating pathogen capture, antigen presentation, and adaptive immune priming while simultaneously serving as an entry portal for viruses such as HIV-1, PRRSV, and SARS-CoV-2, and its interferon-inducible expression correlates with both protective immunity and disease severity in infections, cancer, and chronic inflammatory disorders.

  • 免疫印迹

    • WB result of Sialoadhesin/CD169 Recombinant Rabbit mAb
      Primary antibody: Sialoadhesin/CD169 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated THP-1 whole cell lysate 20 µg
      Lane 2: THP-1 treated with PMA (100 nM, 24 hours), LPS (10 ng/ml, 7 hours）and BFA (5 µg/ml, 5 hours) whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 183 kDa
      Observed MW: 270 kDa

    • WB result of Sialoadhesin/CD169 Recombinant Rabbit mAb
      Primary antibody: Sialoadhesin/CD169 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated RAW264.7 whole cell lysate 20 µg
      Lane 2: RAW264.7 treated with 1 µg/ml LPS for 24 hours whole cell lysate 20 µg
      Lane 3: mouse kidney lysate 20 µg
      Lane 4: mouse spleen lysate 20 µg
      Lane 5: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 183 kDa
      Observed MW: 270 kDa

    • WB result of Sialoadhesin/CD169 Recombinant Rabbit mAb
      Primary antibody: Sialoadhesin/CD169 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat kidney lysate 20 µg
      Lane 2: rat spleen lysate 20 µg
      Lane 3: rat liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 183 kDa
      Observed MW: 270 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human placenta. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse lung. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Sialoadhesin/CD169 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of Raw264.7 cells treated with LPS(1ug/ml,24h) (top panel) and untreated Raw264.7 cells (below panel). Anti- Sialoadhesin/CD169 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Sialoadhesin/CD169 in tumor tissue.

    • Expression of Sialoadhesin/CD169 in human tissue.

    • Expression of Sialoadhesin/CD169 in mouse & rat tissue.

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