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宿主来源
Rabbit抗原名称
AKAP4分子别名
A-kinase anchor protein 4; AKAP-4; A-kinase anchor protein 82 kDa (AKAP 82; hAKAP82); Major sperm fibrous sheath protein (HI); Protein kinase A-anchoring protein 4 (PRKA4); AKAP82细胞定位
Cell projectionAccession
Q5JQC9克隆号
SDT-4196抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:2000 Hu IF 1:500 Hu
AKAP4, the most abundant protein of the sperm fibrous sheath, is a testis-enriched scaffolding protein that simultaneously binds PKA via dual-specificity anchoring sites and PKC through polybasic motifs, thereby acting as a cAMP/PKA ↔ PKC/ERK1/2 switch that is phosphorylated on Ser/Tyr during capacitation to direct the kinase cascade to the flagellar principal piece, mid-piece and post-acrosomal region, enabling progressive motility and male fertility while also moonlighting in multiple cancers where its cytoplasmic/membrane accumulation drives proliferation, EMT and metastasis through the Shh and Wnt/β-catenin pathways.
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human endometrial cancer. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human testis. Anti-AKAP4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of AKAP4 in tumor tissue.
Expression of AKAP4 in human tissue.
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