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宿主来源
Mouse抗原名称
TdT分子别名
DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase (Terminal transferase); TDT; DNTT细胞定位
NucleusAccession
P04053克隆号
SDT-4203抗体类型
Recombinant mAb抗体同种型
IgG2a反应种属 ?
Hu阳性样本
Jurkat, Molt-4, mouse thymus, rat thymus纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:2000 Hu
Terminal deoxynucleotidyl transferase (TdT), also called DNA nucleotidylexotransferase or terminal transferase, is a template-independent DNA polymerase encoded by the DNTT gene on human chromosome 10q23-q24 that is selectively expressed in immature lymphoid precursors (pre-B and pre-T cells) and certain leukemias/lymphomas; it catalyzes the non-templated addition of deoxynucleotides to the 3'-OH ends of DNA, generating N-region diversity at V(D)J junctions during immunoglobulin and T-cell receptor gene rearrangement, thereby expanding antigen-receptor repertoires critical for adaptive immunity, and can utilize a broad range of divalent cations (Mg²⁺, Mn²⁺, Zn²⁺, Co²⁺) as cofactors .
免疫印迹
WB result of TdT Recombinant Mouse mAb
Primary antibody: TdT Recombinant Mouse mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 58 kDaWB result of TdT Recombinant Mouse mAb
Primary antibody: TdT Recombinant Mouse mAb at 1/1000 dilution
Lane 1: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-mouse, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDaWB result of TdT Recombinant Mouse mAb
Primary antibody: TdT Recombinant Mouse mAb at 1/1000 dilution
Lane 1: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-mouse, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human thymus. Anti-TdT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-TdT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human endometrial cancer. Anti-TdT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-TdT antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TdT in tumor tissue.
Expression of TdT in human tissue.
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