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Rabbit抗原名称
CXCL13分子别名
C-X-C motif chemokine 13; Angie; B cell-attracting chemokine 1 (BCA-1); B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; BCA1; BLC; SCYB13免疫原
Recombinant Protein细胞定位
SecretedAccession
O43927克隆号
SDT-2703-132抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Hu IF 1:100 Hu
CXCL13, also called BCA-1 or BLC, is a homeostatic CXC chemokine that acts as the main chemoattractant for B lymphocytes and follicular helper T cells through exclusive binding to the G-protein-coupled receptor CXCR5, thereby guiding their migration into lymphoid follicles and organizing the microarchitecture of secondary lymphoid organs such as lymph nodes and spleen; secreted chiefly by follicular dendritic cells, stromal cells, and macrophages, it triggers downstream signaling cascades including PLC-β/PKCα, MAPK, PI3K-Akt, and NF-κB that promote chemotaxis, adhesion, and survival of B cells, and its dysregulation—manifested by elevated serum or tissue levels—has been mechanistically linked to autoimmune diseases (e.g., rheumatoid arthritis, Sjögren’s syndrome, systemic lupus erythematosus), chronic inflammatory disorders, B-cell malignancies, and neuroinflammatory conditions like multiple sclerosis, making it both a pathogenic driver and a clinically useful biomarker for disease activity and prognosis.
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CXCL13 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CXCL13 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of CXCL13 in tumor tissue.
Expression of CXCL13 in human tissue.
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