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Rabbit抗原名称
NKX3.1分子别名
Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NKX3A; NKX3-1免疫原
Recombinant Protein细胞定位
NucleusAccession
Q99801克隆号
S-2778-63抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
LNCaP, Jurkat, DU 145纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu ICC 1:500 Hu
NKX3.1 is a prostate-specific homeodomain transcription factor that acts as a dose-dependent tumor suppressor by integrating developmental and metabolic cues: in the nucleus it partners with the androgen receptor, FoxA1 and serum response factor to coregulate differentiation genes and a “protein-trafficking” network that includes RAB3B, while in mitochondria it is imported via HSPA9 in response to oxidative stress, binds mtDNA and up-regulates electron-transport-chain genes to restore oxidative phosphorylation, thereby limiting ROS-induced DNA damage; haploinsufficiency, allelic loss or cancer-associated point mutations (e.g., T164A) disrupt these functions, leading to prostatic intraepithelial neoplasia, progression to castration-resistant disease, and reduced survival, whereas high mitochondrial NKX3.1 predicts favorable prognosis, and secreted NKX3.1 can even act as a para-transcription factor to inhibit growth of neighboring cells, illustrating its multifaceted role in prostate development, metabolic homeostasis and cancer suppression.
免疫印迹
WB result of NKX3.1 Recombinant Rabbit mAb
Primary antibody: NKX3.1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: DU 145 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; PC-3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 30 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human prostate. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 1). Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer (case 2). Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-NKX3.1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in LNCaP cells (top panel) and negative staining in PC-3 cells (below panel). Anti-NKX3.1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of NKX3.1 in tumor tissue.
Expression of NKX3.1 in human tissue.
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